Trinity Mirror will close the Liverpool Post next Thursday 19 December. The newspaper's accompanying website and the Liverpool Post Business Daily e-edition will also close on the same day.
The Liverpool Post, which was first published in 1855, had its frequency cut from daily to weekly in January 2012. Elements of the Post's business section will be retained within sister publication the Liverpool Echo.
Steve Anderson, Trinity Mirror North West managing director, said: "Sadly, the Liverpool city region no longer generates the demand in terms of advertising or circulation, to sustain both the Post and the Liverpool Echo."
Mark Thomas, editor at the Liverpool Post, has said that no journalist jobs will be lost as a result of the closure and all will be offered alternative roles at Trinity Mirror.